Subunit From Normal Human Heart
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چکیده
We have cloned and sequenced cDNAs for three isoforms of the L-type voltage-dependent calcium channel subunit isolated from a normal human heart cDNA library. One of these subunits, designated JPa. hybridized with a 3.4-kb message, and the other two, designated Pb and pBc, hybridized with a message of approximately 1.9 kb. The presence of both of these latter two messages in human heart was confirmed by polymerase chain reaction methodology. Considering the differences between P8a and Pb/J3c, we find it likely that these messages may be encoded by two different gene sequences, Pla and Pb/Pc, and that the Pb/Pc sequence can be alternatively spliced in the 209-260 region. The data suggest that the human heart presents a different pattern of P3 subunit expression from that found in the rat and rabbit heart. (Circulation Research 1993;72:1337-1344)
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تاریخ انتشار 2005